《植物生理学报》 2014, 50(4): 419-425

地涌金莲种子形态生理休眠及激素的动态变化

唐安军*

重庆师范大学生命科学学院, 重庆401331

通信作者：唐安军；E-mail: tanganjun@mail.kib.ac.cn；Tel: 023-65910315

摘　要：

珍稀植物地涌金莲在野外能产生大量的种子, 但成熟的种子散布后不能顺利地转化为幼苗。在暖层积(25 ℃)过程中, 蘑菇形的小胚逐渐分化与生长, 6个月后, 胚已形态成熟且能很好地萌发; 同时, 胚内激素ABA含量不断下降, GA₄含量显著增加, 且与胚的形态成熟和萌发力的增强密切相关。这些结果充分证实地涌金莲种子具有形态生理休眠——在很大程度上限制了野外种子萌发。此外, 种子不萌发可能与生境长时间干旱胁迫有关。

关键词：地涌金莲; 暖层积; 脱落酸, 赤霉素; 形态生理休眠; 种子萌发

收稿：2013-11-11   修定：2014-01-20

资助：重庆市科委科技项目(cstc2013jcyjA00002)和重庆师范大学校级科技项目(2011SLX34)。

Morphophysiological Dormancy and Changes of Endogenous ABA and GA4 Contents in Seeds of Musella lasiocarpa

TANG An-Jun*

College of Life Sciences, Chongqing Normal University, Chongqing 401331, China

Corresponding author: TANG An-Jun; E-mail: tanganjun@mail.kib.ac.cn; Tel: 023-65910315

Abstract:

Rare Musella lasiocarpa can naturally produce many seeds that don’t develop into seedlings after shedding. The mushroom-shaped small embryos without obvious differentiation would gradually differentiate and grew in the process of warm stratification at 25 ℃. After 6-month warm stratification, the embryos matured and could germinate well. At the same time, endogenous ABA constantly decreased and however, GA4 markedly increased over time, which was significantly associated with embryo growth and germinability (R2=0.97). Thus, the results fully confirmed that seeds of this species are morphological dormancy, which restricts the germination of M. lasicocarpa seeds to a great extent in the wild. Also, the phenomenon that the seeds cannot germinate in the habitat is closely related with long drought stress in a dry season.

Key words: Musella lasiocarpa (Franch.) C.Y. Wu ex H.W. Li; warm stratification; abscisic acid; gibberellin; morphophysiological dormancy; sexual regeneration